Nuclear Magnetic Resonance (NMR) Spectroscopy is a very versatile technology for the characterization of structure and dynamics of small molecules as well as biological macromolecules in solution, but also in membrane mimicking environments, in solid powders or microcrystals.
CCMSB houses state of the art equipment primarily for solution NMR (Bruker 600, 800 and 900 MHz spectrometers), and a solid state capacity has been added to the Bruker 800 MHz spectrometer. A typical aqueous sample consists of a 200-500 μL solution with 10μM to > 1mM of the relatively pure molecule of interest. In optimal cases the technique can be used to obtain structural information up to 60-200 kDa molecular weight. Structure determination, characterization of molecular dynamics and protein-ligand or protein-protein interactions are more routine in the sub-30 kDa range. For most applications, it is necessary to obtain isotope-labeled forms of the molecule to facilitate the high- resolution spectral analysis (15N and/or 13C labelling). Thus most proteins are expressed in bacteria or other cells which can be grown in isotope enriched media.
We encourage investigators to contact NMR CCMSB members to obtain assistance and support for pilot projects, and to inquire about possible collaborations. Training for independent use of the instruments is mandatory, but assisted use can also be provided with hourly fees by facility manager, Dr. Xi-an Mao.
Xi-an Mao, Ph.D.
NMR Facility Manager
Case Western Reserve University
Cleveland Center for Membrane and Structural Biology
1819 E 101st St
Cleveland, Ohio 44106-7099